Figure 1 | Scientific Reports

Figure 1

From: Hepatic stem cell Numb gene is a potential target of Huang Qi Decoction against cholestatic liver fibrosis

Figure 1

Lentivirus-mediated knockdown of the BMSC Numb gene. (a) Flow cytometry analysis of CD10, CD14, CD29, CD34, CD45, and CD90. (b) Flow cytometry analysis of BMSC proliferative capacity. (c) Osteogenic induction (×200). (d) Adipogenic induction (×200). (e) Lentivirus transfected BMSCs. (f) The relative Numb mRNA level determined by qRT-PCR, mRNA level were normalized by GAPDH (n = 3 per group). (g) Immunobloting for Numb, and (h) The gray-level score indicates the histogram of immunoblotting for Numb (n = 3 per group). *P < 0.05, **P < 0.01. BMSC, bone marrow-derived mesenchymal stem cell group; NC, empty virusgroup; Numb-KD, Numb knockdown group.

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