Figure 3
BMSCNumb-KD transplantation reduces the inhibitory effect of HQD on bile duct proliferation. (a) Immunostaining of CK7 (×200). (b) Immunostaining of CK19 (×200). (c) Immunostaining of HNF4α (×200). (d) Positive area percentage of CK7 and CK19 immunostaining (n = 5 per group). (e) Immunoblotting of CK7 and CK19, and (f) The gray-level score indicates the histogram of immunoblotting for CK7 and CK19 (n = 5 per group). (g) Relative CK19, CK7 and HNF4α expression levels measured by qRT-PCR, mRNA levels were normalized by GAPDH (n = 5 per group). (h) Double immune-staining of CK7 (red) and EGFP (green) (×400). (i) Double immune-staining of CK19 (red) and EGFP (green) (×400). *P < 0.05, **P < 0.01. Sham, sham group; BDL, bile duct ligation group; HQD, Huang Qi decoction group; DAPT, DAPT group; BMSCNC, BMSC negative control group; BMSCNumb-KD, BMSC Numb knockdown group; and HQD + BMSCNumb-KD, HQD plus BMSCNumb-KD group.
