Figure 6 | Scientific Reports

Figure 6

From: Hepatic stem cell Numb gene is a potential target of Huang Qi Decoction against cholestatic liver fibrosis

Figure 6

Numb knockdown reduces the inhibitory effect of HQD on Notch signal pathway activation in WB-F344. (a) Immunostaining of Numb (×600). (b) Immunostaining of RBP-Jκ (×600). (c) Immunostaining of Hes1 (×600). (d) Immunoblotting for Numb, RBP-Jκ and Hes1, and (e) The gray-level score indicates the histogram of immunoblotting for Numb, RBP-Jκ and Hes1 (n = 3 per group). (f) Relative levels of Numb, RBP-Jκ and Hes1 measured by qRT-PCR, and mRNA levels were normalized by GAPDH (n = 3 per group). *P < 0.05, **P < 0.01. N, normal group; SB, sodium butyrate group; HQD, SB plus Huang Qi decoction group; DAPT, SB with DAPT group; NC, empty virus with SB group; Numb-KD, Numb knockdown with SB group; and HQD + Numb-KD, Numb knock down and SB with Huang Qi Decoction group.

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