Table 1 Primer pairs and probes used for real-time PCR.
Gene | Primer sequence | Note | |
|---|---|---|---|
Notch1 | Forward Reverse | TGGATGAGGAAGACAAGCATTA GAAAAGCCACCGAGATAGTCAG | SYBR |
Notch2 | Forward Reverse | GAG GAA GAA GTG TCT CAA GTG GCA TCA GAA ACA TAT G | SYBR |
Notch3 | Forward Reverse | GAC AAG GAC CAC TCC CAC TACT ATC CAC ATC ATC CTC ACA ACT G | SYBR |
Notch4 | Forward Reverse | TGT CAG GAA CCA GTG TCA GAA C CCT GGG CTT CAC ATT CAT CTA T | SYBR |
Jagged1 | Forward Reverse | CCA TCA AGG ATT ATG AGA AC TGG TGC TTA TCC ATA TCA | SYBR |
Jagged2 | Forward Reverse | AAA TGA GTG GTC CGT GGC AGA TGG TTG GAA GCC TTG TCT GCT | SYBR |
Delta 1 | Forward Reverse | GTG TGC AGA TGG TCC TTG CTT C CTG ACA TCG GCA CAG GTA GGA G | SYBR |
Delta 4 | Forward Reverse | GCA GAA CCA CAC ACT GGA CTA T TGG CAC CTT CTC TCC TAA ACT C | SYBR |
Numb | Forward Reverse | GCT ACT TTC GAT GCC AGT AGA ACC A CTG TTG CCA GGA GCC ACT GA | SYBR |
Hes1 | Forward Reverse | GAC GGC CAA TTT GCT TTC GAC ACT GCG TTA GGA CCC | SYBR |
RBP-Jκ | Forward Reverse | TTG CTT ACC TTC AGG CGT GTG GCC CAA TGA GTC TGC TGC AA | SYBR |
α-SMA | Forward Reverse | AAT GGC TCT GGG CTC TGT AA TCT CTT GCT CTG GGC TTC AT | SYBR |
Collagen I | Forward Reverse | ACG TCC TGG TGA AGT TGG TC TCC AGC AAT ACC CTG AGG TC | SYBR |
CollagenIV | Forward Reverse | TTT CCA GGG TTA CAA GGT GT AGT CCA GGT TCT CCA GCA TC | SYBR |
TGF-β1 | Forward Reverse | ATT CCT GGC GTT ACC TTG G AGC CCT GTA TTC CGT CTC CT | SYBR |
TNF-α | Forward Reverse | GAC GTG GAA CTG GCA GAA GAG TTG GTG GTT TGT GAG TGT GAG | SYBR |
CK7 | Forward Reverse | AGG AAC AGA AGT CAG CCA AGA G GCA ACA CAA ACT CAT TCT CAG C | SYBR |
CK19 | Forward Reverse | GAT CTG CGT AGT GTG G AAA ACC AAA CTG GGG ATG | SYBR |
LNX1 | Forward Reverse | TGC TGC CAG GAG ACA TCA T CAT TGC TTC TGC TAC GGA ACT T | SYBR |
LNX2 | Forward Reverse | ACA CAG ATT GAG GGT GAA ACT GGT CCA CAC AGG AAG AGG T | SYBR |
ITCH | Forward Reverse | ATG GGA GAT TTG TCA GTT TGT C CAG CGT CAT TCT GTG TAG CA | SYBR |
GAPDH | Forward Reverse | GGC ACA GTC AAG GCT GAG AAT G ATG GTG GTG AAG ACG CCA GTA | SYBR |
