Figure 5
Compounds 8i and 11 decrease HUVEC tube length in vitro. In vitro tube forming assay, and quantification of tube length was performed as described under “Materials and methods”. Briefly, HUVEC cells were seeded on top of matrigel and incubated for 4 h to form tubes following VEGF stimulation. Cells were then treated with 1 µM and 5 µM of compound 8i and 11 for 12 h. The fold changes were calculated by comparing the tube length of treated versus non-treated cells. Statistical significance (Kruskal–Wallis one-way ANOVA) of tube length inhibition by compound 8i and 11 was compared to the non-treated cells. All images were captured using the same magnification and are representative of 10 fields of view (see scale bar, 250 µm). Bars indicate Mean + SD of 30 independent tube length measurements (n = 30, ****P < 0.0001).
