Figure 2
From: Loss of PRCD alters number and packaging density of rhodopsin in rod photoreceptor disc membranes
Reduced rod photoreceptor function and slow retinal degeneration in animals lacking Prcd. (A) Representative waveform from scotopic ERGs of WT (black) and Prcd-KO (red) animals at P30 (0.995 cd*s/m2). (B) Significant loss of rod photoreceptor function at higher light intensities (0.158, 0.995, and 2.5 cd*s/m2) compared with low light conditions (0.00025 and 0.001 cd*s/m2) (n = 4, data from B and C stats are unpaired two-tailed t-test; higher light intensities were statistically significant, *p < 0.01; Low light intensities were not significant). (C) Maximum a-wave amplitude of Prcd-KO animals at different ages compared to WT controls from P30 to P200. (D) Representative waveform from photopic (cone) ERGs of Prcd-KO and WT animals at P200 (7.9 cd*s/m2) (n = 4). (E) Prcd-KO and WT littermate control cross-sections stained with hematoxylin and eosin (H&E), imaged by light microscopy, at P30, P120, and P250. Scale bar = 20 μm. (F) Quantification of number of photoreceptor nuclei in the outer nuclear layer (ONL) from both Prcd-KO (red) and WT littermate controls (black), every 350 μm from the optic nerve, at P30, P120, and P250. Data are represented as mean (n = 3, unpaired two-tailed t-test; *p < 0.01).
