Figure 1

Survival analyses in ccRCC (TCGA) based on HIF1A CNV, HIF1A mRNA expression, HIF1α protein and CA9 protein expression. (A) Lower HIF1A CNV (< -0.0297 log2(copy-number/2)) in primary ccRCC tumors was associated with worse overall survival than higher HIF1A CNV (≥ -0.0297 log2(copy-number/2)) (log-rank test p = 0.01, n = 531, TCGA clear cell RCC). (B) However, there was no significant difference in survival between ccRCC patients with lower HIF1A mRNA expression in primary tumors (< 19.4 log2(fpkm-uq + 1)) and higher HIF1A mRNA expression (≥ 19.4 log2(fpkm-uq + 1)) (log-rank test p = 0.33, n = 530, TCGA clear cell RCC). Fpkm-uq (Fragments per kilobase of transcript per million mapped reads- upper quartile) is an RNA-sequencing-based expression normalization method. (C) Similarly, no significant survival difference between ccRCC patients with lower HIF1α protein expression and higher HIF1α protein expression based on Reverse Phase Protein Array functional proteomics (log-rank test p = 0.4, n = 444, The Cancer Proteome Atlas). (D) Also, no significant survival difference between ccRCC patients with lower CA9 protein expression and higher CA9 protein expression (log-rank test p = 0.4, n = 444). In fact, the curves separated beyond ~ Day 2500 and at Day 4000, the higher CA9 expression group had a lower survival rate of 28% compared to that of 51% in the lower CA9 expression group.