Figure 3
From: HIF1α is not a target of 14q deletion in clear cell renal cancer
(A) Genetic loss (shallow deletions) of HIF1A was associated with a much greater reduction of L2HGDH expression than its own gene expression (TCGA ccRCC, cbioportal). (B) Survival based on L2HGDH expression with the same dataset (TCGA ccRCC, n = 530). Higher L2HGDH expression (≥ 15.16 log2(fpkm-uq + 1)) was associated with significantly improved survival (log-rank p value as depicted). (C) In ccRCC patients with complete (uncensored) survival data (TCGA), patients with “HIF1A shallow deletion + L2HGDH diploid” had significantly greater mean survival (1670.8 days + /- 183.5 days, n = 5) compared to patients with “HIF1A shallow deletion + L2HGDH shallow deletion” (885.1 days + /- 74.3 days, n = 87) (t-test p value = 0.007). Data represented as means ± s.e.m. (D) Kaplan Meier survival curves of the two groups showing a wide separation between the curves (Peto and Peto modification of the Gehan- Wilcoxon test ‘p’ value = 0.033). Put together, when genetic loss of HIF1A occurs without genetic loss of L2HGDH in ccRCC, the survival is significantly greater than when there is simultaneous genetic loss of HIF1A and L2HGDH, which argues against HIF1A being a target of 14q deletion, and against HIF1A being a 14q tumor-suppressor in ccRCC.
