Figure 3 | Scientific Reports

Figure 3

From: Pathogenesis of CDK8-associated disorder: two patients with novel CDK8 variants and in vitro and in vivo functional analyses of the variants

Figure 3

Results of CDK8 mRNA overexpression analyses. (a) Photographs of zebrafish embryos at the 1-day post-fertilization (dpf) stage. An uninjected embryo was used as a control. The wild-type CDK8 mRNA-injected embryo exhibited severe defects. Note that the CDK8 mRNA injection induced a shortened body axis, small anterior structures, and an aberrant tail morphology. Scale bar, 500 μm. (b) Graph showing the proportions of abnormal zebrafish embryos. The error bars represent the SD for three independent experiments (for uninjected: n = 15, 36, 63 for CDK8: n = 34, 35, 31 for CDK8-G28A: n = 26, 65, 63 for CDK8-N156S: n = 27, 23, 28), *p = 0.02117, ***p = 6.224e−13 using a chi-square test.

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