Figure 7
Glucagon treatment of U neonates fails to induce autophagy-dependent mobilization of liver glycogen. C and U neonates were treated or not with a single intraperitoneal dose of rapamycin (RP; 5 mg/kg) or glucagon (GN; 20 mg/kg) at birth and then 3 h-fasted (N3) before measurement of (A) liver glycogen content or (B) basal glycemia, n = 6–12. All values are mean ± SD. *p < 0.05 U versus C neonates within the same treatment. #p < 0.05 RP-treated or GN-treated versus untreated N3 neonates within the same nutritional condition. (C) Protein extracts from livers of C and U neonates at N3, RP- or GN-treated, were immunoblotted for autophagy markers LC3B and p62. Graphical bars represent densitometric quantification relative to β-actin protein levels from 4 individual animals per group. All values are mean ± SD. *p < 0.05 U versus C neonates under the same treatment.
