Table 2 Kinetic parameters of LarA and 7 LarAHs.
From: Uncovering a superfamily of nickel-dependent hydroxyacid racemases and epimerases
Substrate | pH opta | Temp opta (°C) | KM (mM)b | kcat (s−1)b | kcat/KM (s−1 mM−1) | |
|---|---|---|---|---|---|---|
LarA17 | L-lactate | 6 | 35–40 | 46 ± 20 | 4,750 ± 500 | 103 ± 50 |
D-lactate | 11 ± 4 | 1,300 ± 150 | 121 ± 56 | |||
LarAH2/Sar | L-lactate | 7.5 | 45–50 | 0.15 ± 0.04 | 4.7 ± 0.3 | 31 ± 10 |
D-lactate | 0.56 + 0.10 | 8.1 ± 0.5 | 14 ± 4 | |||
LarAH5/Mar1 | L-malate | 9 | 45–50 | 55 ± 6 | 140 ± 9 | 0.39 ± 0.07 |
LarAH6/Mar2 | L-malate | 6 | 35–45 | 0.38 ± 0.04 | 66 ± 10 | 185 ± 49 |
LarAH7/Hgr | L-hydroxy-glutarate | 7–8 | NI | 0.32 ± 0.06 | > 1.5 | > 4.7 ± 1 |
LarAH10/Plr | L-phenyl-lactic acid | NI | NI | 0.4 ± 0.3 | 0.16 ± 0.03 | 0.28 ± 0.21 |
LarAH19/GntE1 | D-mannonate | 5.5–8 | 30–55 | 120 ± 23 | 59 ± 12 | 0.48 ± 0.15 |
LarAH20/GntE2 | D-mannonate | 6 | 80–90 | 17 ± 6 | 197 ± 20 | 11 ± 5 |
