Table 2 Primers used in qRT-PCR primer assays.

From: Isolation and enrichment of melanocytes from human corneal limbus using CD117 (c-Kit) as selection marker

Gene symbol

Accession no.

Product length

Probe no.

Sequence 5′–3′

GAPDH

NM_002046.3

72

P3

CAGCAAGAGCACAAGAGGAA

GTGGTGGGGGACTGAGTGT

KIT

NM_000222.2

61

P6

GAGTAGCTTACCAGAAGCTTCCATAG

CATAGGGACTGATGCCTTCC

MLANA

NM_005511.1

79

P39

GAGAAAAACTGTGAACCTGTGGT

AAGGTGGTGGTGACTGTTCTG

TYRP1

NM_000550.2

77

P2

CCTGTGACCAGAGGGTTCTC

CCGGACAAAGTGGTTCTTTTC

  1. Primers were used for Probe based (Universal Probe Library) qRT-PCR assays with an annealing temperature of 60 °C.
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