Table 3 List of antibodies used.
Antibody (clone), host species | Antibody concentration | Application | Antibody source |
|---|---|---|---|
CD117 (c-Kit), APC (A3C6E2), mouse | 10 µl/106 cells | Flow cytometry | Miltenyi Biotec |
IgG3 isotype FITC (MG3-35), mouse | 5 µg/ml | Flow cytometry | Biolegend |
IgG1 isotype APC (MOPC-21), mouse | 20 µl/106 cells | Flow cytometry | BD |
Melan-A FITC, (MLANA/788), mouse | 5 µg/ml | Flow cytometry | Novus Biologicals |
Cytokeratin 3 (AE5), mouse | 1:100 | Immunohistochemistry | Abcam |
Cytokeratin 15 (LHK15), mouse | 1:500 | Immunohistochemistry | Abcam |
Cytokeratin 15 (EPR1614Y), rabbit | 1:500 | Immunohistochemistry | Abcam |
Vimentin, (D21H3), rabbit | 1:500 | Immunohistochemistry | Cell Signaling |
HMB-45 (HMB45), mouse | 0.5 µg/ml | Immunocytochemistry | Abcam |
Sox10 (EPR4007), rabbit | 1:250 | Immunocytochemistry | Abcam |
TRP1 (EPR21960), rabbit | 1:1000 | Immunocytochemistry | Abcam |
CD117/c-kit (K45), mouse | 1:100 | Immunohisto/cytochemistry | Invitrogen |
Mouse IgG1k | 100 µg/ml | Immunohisto/cytochemistry | BD |
Melan-A (OTI3E2), mouse | 1:100 | Immunohisto/cytochemistry | ORIGENE |
GAPDH; (6C5), mouse | 1:50,000 | Western blot | Millipore |
CD117/c-Kit (Ab81), mouse | 1:500 1.1000 | Immunohisto/cytochemistry, Western blotting | Cell Signaling |
Melan A, (EPR20380), rabbit | 1:1000 1:10,000 | Immunohisto/cytochemistry, Western blotting | Abcam |
