Figure 2

Macroscopic and microscopic anatomy of testes in 16-month-old male wild type, rescued and germ cell-free dnd crispant Atlantic salmon. The gross morphology and histology of a control male is shown on the first row (a, d); the middle row shows a dnd crispant male with germ cells rescued by dnd mRNA injection (b, e); the last row shows a dnd crispant GCF albino male (c, f). The testis of each males is shown by white arrows (a, b, c); The spermatogonia (Spg; black arrowhead) surrounded by Sertoli cells (Sc; black arrow) were observed in testis of the control wt male (d) and the rescued dnd crispant male (e) but not in testis of dnd crispant albino male (f). Wt control male: 162, rescued male: 148, GCF male: 16.