Figure 2

PQ influenced the proliferation, but not apoptosis of NSC in SGZ and SVZ. B6 mice were treated with PQ twice with 1wk apart. Thereafter, the cell suspensions from SGZ and SVZ were prepared to detect the expression of cleaved (active) Caspase-3, Bcl-2, and Ki67 by flow cytometry. The slices were prepared for immunohistochemistry staining. a, b The mean fluorescence intensity (MFI) of cleaved Caspase-3 of NSC in SGZ (a) and SVZ (b). c, d The MFI of Bcl-2 of NSC in SGZ (c) and SVZ (d). e, f Representative flow plots for the proliferation (Ki67⁺) of NSC in SGZ (e) and SVZ (f). g, h Representative confocal images of proliferation of NSC (SOX2⁺Ki67⁺) in SGZ (g) and SVZ (h); Ki67, SOX2 and DAPI were in green, yellow and blue respectively; SOX2⁺Ki67⁺cells were marked by white arrows; scale bars = 50 μm. Asterisk (*) indicates a significant difference (*p < 0.05, one-way ANOVA) and the n.s. means no difference as indicated. 6 mice were used for each group.