Figure 5

TUDCA alleviated the neuroinflammation and NSC impairments. B6 mice were treated with vehicle control, 20 mg/kg PQ or 20 mg/kg PQ + TUDCA; the inflammation cytokines were detected by ELISA; the NSCs were measured by flow cytometry and immunohistochemistry. a, b The concentrations of IL-6 (left), IL-1β (middle) and TNF-α (right) in SGZ (a) and SVZ (b). c, d Representative confocal images of NSC (DAPI⁺SOX2⁺) in SGZ (c) and SVZ (d); DAPI and SOX2 were in blue and yellow, respectively; the representative images of NSC were marked by the black arrows; all the black-green dots represent the NSC; scale bars = 50 μm. e, f The percentages of NSC (CD45⁻SOX2⁺ cells) in SGZ (e) and SVZ (f). Asterisk (*) indicates a significant difference (*p < 0.05, one-way ANOVA) and the n.s. means no difference as indicated. 6 mice were used for each group.