Figure 6 | Scientific Reports

Figure 6

From: Interleukin-11 is important for vascular smooth muscle phenotypic switching and aortic inflammation, fibrosis and remodeling in mouse models

Figure 6

Anti-IL11 antibodies reduce TAC-induced molecular changes in the mouse aorta. (a) RT-qPCR of Il11 mRNA expression normalized to Gapdh expression in aortas of Sham, TAC + IgG or TAC + X203 mice (n = 4/group). (b) Representative immunoblots of IL11, ACTA2, COL3A1, and GAPDH in the aorta of Sham, TAC + IgG or TAC + X203 treated animals (n = 3/group). Collated densitometry for IL11, ACTA2 and COL3A1 normalized to GAPDH protein expression. Samples were derived from the same experiment and blots were processed in parallel. Extended blots are presented in Supplementary Fig. S14 online. Data expressed in mean ± SD. RT-qPCR of (c) contractile genes (Acta2, Cnn1, Myh11, Myocd*, and Tagln), (d) ECM genes (Col1a1, Col1a2, Col3a1, Fn1, Mmp2, and Timp1), and (e) inflammatory genes (Il6, Tnf, Ccl2 and Ccl5) normalized to Gapdh expression in Sham, TAC + IgG or TAC + X203 mice (n = 3–4/group). Statistical analyses by one-way ANOVA with Sidak multiple comparisons unless data deviated from normal, in which case a Kruskal–Wallis test was performed (denoted by *). Data presented as median ± IQR, whiskers demarcate the minimum and maximum values.

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