Table 1 Activity rhythms of RNAi knockdown lines that target Ubc6, Prl-1 and Nipped-A.

From: Proteomic analysis of Drosophila CLOCK complexes identifies rhythmic interactions with SAGA and Tip60 complex component NIPPED-A

Genotype

Total

% Rhythmic

Period ± s.e.m

Strength ± s.e.m

w1118

16

100

23.64 ± 0.06

721.71 ± 74.91

w1118, UAS-dcr; tim-Gal4/ + ; + / + 

15

100

24.50 ± 0.13

673.16 ± 64.50

w1118, UAS-dcr; + / + ; pdf-Gal4/ + 

16

100

24.60 ± 0.08

680.41 ± 58.88

w1118, UAS-dcr; Ubc6 RNAia/tim-Gal4; + / + 

28

100

24.43 ± 0.12

201.61 ± 25.27

w1118, UAS-dcr; Ubc6 RNAi/ + ; pdf-Gal4/ + 

17

100

23.81 ± 0.05

340.92 ± 45.96

w1118; Ubc6 RNAi/ + ; + / + 

19

100

23.22 ± 0.20

245.08 ± 39.25

w1118, UAS-dcr; tim-Gal4/ + ; PRL-1 RNAib/ + 

9

44

24.97 ± 1.84

12.18 ± 4.194

w1118, UAS-dcr; + / + ; PRL-1 RNAi/pdf-Gal4

16

75

26.36 ± 0.581

109.78 ± 47.535

w1118; + / + ; PRL-1 RNAi/ + 

15

100

23.38 ± 0.06

866.26 ± 92.22

w1118, UAS-dcr; Nipped-A RNAi1c/tim-Gal4; + / + 

14

93

26.06 ± 0.132

295.93 ± 49.94

w1118, UAS-dcr; Nipped-A RNAi1/ + ; pdf-Gal4/ + 

14

93

26.49 ± 0.233

283.61 ± 48.25

w1118; Nipped-A RNAi1/ + ; + / + 

16

94

24.00 ± 0.22

329.50 ± 60.17

  1. Activity rhythm period in constant darkness is given in hours ± standard error of the mean (s.e.m.).
  2. aUbc6 RNAi, BDSC# 42,631.
  3. bPRL-1 RNAi, BDSC# 38,358.
  4. cNipped-A RNAi1, VDRC #GD9847. The sites targeted by Nipped-A RNAi1 is described in Fig. 2.
  5. 1Significantly longer than w1118, UAS-dcr; + / + ; pdf-Gal4/ + control flies (p < 10–3).
  6. 2Significantly longer than w1118, UAS-dcr; tim-Gal4/ + ; + / + control flies (p < 10–6).
  7. 3Significantly longer than w1118, UAS-dcr; + / + ; pdf-Gal4/ + control flies (p < 10–8).
  8. 4Significantly lower power than w1118, UAS-dcr; tim-Gal4/ + ; + / + control flies (p < 10–7).
  9. 5Significantly lower power than w1118, UAS-dcr; + / + ; pdf-Gal4/ + control flies (p < 10–6).
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