Table 2 Activity rhythms of RNAi knockdown lines that target different portions of Nipped-A transcript.

From: Proteomic analysis of Drosophila CLOCK complexes identifies rhythmic interactions with SAGA and Tip60 complex component NIPPED-A

Genotype

Total

% Rhythmic

Period ± SEM

Strength ± SEM

w1118

16

100

23.46 ± 0.05

630.71 ± 68.88

w1118, UAS-dcr; tim-Gal4/ + ; + / + 

16

100

24.45 ± 0.09

331.74 ± 51.95

w1118, UAS-dcr; + / + ; pdf-Gal4/ + 

14

100.00

24.06 ± 0.10

402.85 ± 71.09

w1118, UAS-dcr; tim-Gal4/ + ; Nipped-A RNAi2a/ + 

12

91.66

27.20 ± 0.201

300.95 ± 47.67

w1118, UAS-dcr; Nipped-A RNAi2/ pdf-Gal4

15

100.00

25.81 ± 0.192

462.04 ± 42.47

w1118; Nipped-A RNAi2/ + 

16

87.50

24.08 ± 0.12

366.06 ± 50.62

w1118, UAS-dcr; Nipped-A RNAi3b/ tim-Gal4

15

100.00

26.31 ± 0.123

300.64 ± 43.49

w1118, UAS-dcr; + / + ; Nipped-A RNAi3/ pdf-Gal4

13

100.00

25.51 ± 0.394

247.20 ± 37.26

w1118; Nipped-A RNAi3/ + 

16

100.00

23.89 ± 0.03

1008.67 ± 125.11

  1. Activity rhythm period in constant darkness is given in hours ± standard error of the mean (s.e.m.).
  2. aNipped-A RNAi2, VDRC #GD15595.
  3. bNipped-A RNAi3, NIG# 2905R-7. The sites targeted by Nipped-A RNAi2 and Nipped-A RNAi3 are described in Fig. 2.
  4. 1Significantly longer than w1118, UAS-dcr; tim-Gal4/ + ; + / + control flies (p < 10–30).
  5. 2Significantly longer than w1118, UAS-dcr; + / + ; pdf-Gal4/ + control flies (p < 10–11).
  6. 3Significantly longer than w1118, UAS-dcr; tim-Gal4/ + ; + / + control flies (p < 10–33).
  7. 4Significantly longer than w1118, UAS-dcr; + / + ; pdf-Gal4/ + control flies (p < 10–3).
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