Table 3 Activity rhythms of RNAi knockdown lines that target components of SAGA, Tip60 and Xmas-2 complexes.

From: Proteomic analysis of Drosophila CLOCK complexes identifies rhythmic interactions with SAGA and Tip60 complex component NIPPED-A

Genotype

Total

% Rhythmic

Period ± SEM

Strength ± SEM

w1118

16

81

23.50 ± 0.09

459.00 ± 93.23

w1118, UAS-dcr; tim-Gal4/ + ; + / + 

16

100

24.63 ± 0.15

679.73 ± 83.96

w1118; UAS-dcr/ + ; tim-Gal4/ + 

16

100

24.33 ± 0.08

690.68 ± 67.32

w1118, UAS-dcr; + / + ; + /pdf-Gal4

16

100

24.60 ± 0.06

706.62 ± 104.19

w1118, UAS-dcr; tim-Gal4/ + ; Ada2b RNAia/ + 

16

93.75

25.44 ± 0.201

246.41 ± 37.95

w1118, UAS-dcr; + / + ; Ada2b RNAi/pdf-Gal4

16

93.75

25.46 ± 0.242

222.36 ± 47.13

w1118; + / + ; Ada2b RNAi/ + 

16

100.00

24.12 ± 0.10

416.14 ± 48.76

w1118, UAS-dcr; Sgf29 RNAib/tim-Gal4; + / + 

16

100.00

24.96 ± 0.21

325.04 ± 54.82

w1118, UAS-dcr; Sgf29 RNAi/ + ; + /pdf-Gal4

15

93.33

25.12 ± 0.35

180.46 ± 50.50

w1118; Sgf29 RNAi/ + ; + / + 

16

100.00

23.78 ± 0.10

391.31 ± 37.40

w1118, UAS-dcr; Gcn5 RNAic/tim-Gal4; + / + 

16

93.75

25.34 ± 0.33

155.12 ± 43.72

w1118, UAS-dcr; Gcn5 RNAi/ + ; + /pdf-Gal4

10

90.00

24.77 ± 0.66

123.96 ± 48.11

w1118; RNAi Gcn5 RNAi/ + ; + / + 

15

100.00

23.60 ± 0.12

242.17 ± 29.91

w1118, UAS-dcr; Tip60 RNAid/tim-Gal4; + / + 

30

40.00

25.35 ± 0.94

19.95 ± 5.147

w1118, UAS-dcr; Tip60 RNAi/ + ; + /pdf-gal4

32

53.12

24.30 ± 0.56

27.66 ± 6.148

w1118; + / + ; Tip60 RNAi/ + 

31

96.77

23.92 ± 0.09

214.56 ± 23.35

w1118, UAS-dcr; not RNAie/tim-Gal4; + / + 

14

80.00

25.92 ± 1.13

48.27 ± 20.88

w1118, UAS-dcr; not RNAi/ + ; + /pdf-Gal4

14

78.57

26.42 ± 0.803

42.38 ± 8.54

w1118; not RNAi/ + ; + / + 

16

100.00

24.01 ± 0.08

198.57 ± 33.57

w1118, UAS-dcr; e(y)2 RNAif/tim-Gal4; + / + 

16

87.50

26.12 ± 0.584

52.88 ± 10.219

w1118; UAS-dcr; e(y)2 RNAi/ + ; + /pdf-Gal4

16

93.75

25.84 ± 0.425

119.98 ± 27.0710

w1118; e(y)2 RNAi/ + ; + / + 

16

93.75

23.74 ± 0.14

397.22 ± 64.22

w1118, UAS-dcr; xmas RNAig/tim-Gal4; + / + 

14

92.85

24.84 ± 0.78

50.38 ± 6.66

w1118, UAS-dcr; + / + ; xmas RNAi/pdf-Gal4

31

100.00

23.90 ± 0.12

168.75 ± 22.44

w1118, xmas RNAi/ + ; + / + 

16

93.75

23.47 ± 0.28

181.43 ± 40.95

w1118; UAS-dcr/ + ; Sem1 RNAih/tim-Gal4

2*

50

30.80 ± 0.00

15.12 ± 5.17

w1118, UAS-dcr; + / + ; Sem1 RNAi/pdf-Gal4

16

81.25

25.65 ± 0.196

156.36 ± 27.69

w1118; + / + ; Sem1 RNAi/ + 

16

93.75

24.24 ± 0.42

358.74 ± 52.93

  1. Activity rhythm period in constant darkness is given in hours ± standard error of the mean (s.e.m.).
  2. *14 of 16 flies died during the behavioral experiment.
  3. aAda2b RNAi1, NIG# 9638R-3.
  4. bSgf29 RNAi, NIG# 10509R-1.
  5. cGcn5 RNAi, BDSC# 9332.
  6. dGcn5 RNAi, NIG 6121R1.
  7. enot RNAi, NIG# 4166-R1.
  8. fe(y)2 RNAi; VDRC# KK102036.
  9. gxmas RNAi, BDSC# 44427.
  10. hSem1 RNAi, BDSC# 28057.
  11. 1Significantly longer than w1118, UAS-dcr; tim-Gal4/ + ; + / + control flies (p < 10–2).
  12. 2Significantly longer than w1118, UAS-dcr; + / + ; pdf-Gal4/ + control flies (p < 10–2).
  13. 3Significantly longer than w1118, UAS-dcr; + / + ; pdf-Gal4/ + control flies (p < 10–2).
  14. 4Significantly longer than w1118, UAS-dcr; tim-Gal4/ + ; + / + control flies (p < 0.05).
  15. 5Significantly longer than w1118, UAS-dcr; + / + ; pdf-Gal4/ + control flies (p < 10–2).
  16. 6Significantly longer than w1118, UAS-dcr; + / + ; pdf-Gal4/ + control flies (p < 0.05).
  17. 7Significantly lower power than w1118, UAS-dcr; tim-Gal4/ + ; + / + control flies (p < 0.05).
  18. 8Significantly lower power than w1118, UAS-dcr; + / + ; pdf-Gal4/ + control flies (p < 10–2).
  19. 9Significantly lower power than w1118, UAS-dcr; tim-Gal4/ + ; + / + control flies (p < 10–2).
  20. 10Significantly lower power than w1118, UAS-dcr; + / + ; pdf-Gal4/ + control flies (p < 0.05).
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