Figure 5

Effects of inhibitors of ABC transporters on KP-1 staining. (A) Cyclosporine A treatment. miTS-P cells 5 days after passage were treated with 2 µM KP-1 for 3 h at 37 °C. The cells were then cultured with the Abcc10 inhibitor cyclosporine A (5 µM) for 2 h, then treated with 2 µM KP-1 for 3 h at 37 °C. (B) Sildenafil treatment. miTS-P cells 5 days after cell passage were treated with 2 µM KP-1 for 3 h at 37 °C. The cells were then cultured with the Abcc5 inhibitor sildenafil (50 µM) for 2 h and then treated with 2 µM KP-1 for 3 h at 37 °C. (C–G) Treatment with siRNAs specific for cognate ABC transporters. miTS-P cells 5 days after passage were treated with 2 µM KP-1 for 3 h at 37 °C. The cells were then transfected with each siRNA of ABC transporter and cultured for 24 h, then treated with 2 µM KP-1 for 3 h at 37 °C. (C) Treatment with Abcc10-siRNA. (D) Treatment with Abcc12-siRNA. (E) Treatment with Abcc5-siRNA. (F) Treatment with Abca2-siRNA. (G) Treatment with control siRNA. Scale bar = 200 µm.