Figure 1 | Scientific Reports

Figure 1

From: Loss of RBMS1 as a regulatory target of miR-106b influences cell growth, gap closing and colony forming in prostate carcinoma

Figure 1

RBMS1 as putative target for miR-106b is downregulated in PCa cell lines and primary prostate tumour tissue. (A) The predicted binding site for miR-106b inside the RBMS1 3′UTR including the mutation site of its seed sequence and (B) the secondary structure of the miR-106b-RBMS1 hybridization created using RNAhybrid online tool (https://bibiserv.cebitec.uni-bielefeld.de/rnahybrid). (C) Expression levels of RBMS1 in DU145 and LNCaP cells relative to primary PNF-08 cells. Total mRNA was extracted and the relative levels of RBMS1 was determined by qRT-PCR. Levels were quantified relative to the amounts observed in PNF-08 cells. (D) In total RNA, extracted from 5 pairs of primary CaP (tumor) and corresponding non-tumor (normal) prostate tissues, the relative expression of RBMS1 was determined by qRT-PCR. The data is shown as mean ± SEM performed in triplicates.

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