Figure 5 | Scientific Reports

Figure 5

From: Loss of RBMS1 as a regulatory target of miR-106b influences cell growth, gap closing and colony forming in prostate carcinoma

Figure 5

Reduction of gap closing by RBMS1 in PCa cells. DU145 (A,C) or LNCaP cells (E,G) were transfected either with RBMS1 expression vectors or RBMS1 targeting siRNAs and their corresponding controls, respectively, and cells were seeded into a ibidi Culture-Insert system to create a defined cell-free gap. Gap closing was monitored for 36 h (DU145) or 96 h (LNCaP) in three independent experiments. For each experiment, the gap width after removing the ibidi culture insert at 0 h was normalized to 0% and three independent visual fields per time point and replicate were examined (*p < 0.05; ***p < 0.001). (B, D, F, H) Representive pictures at different time points are depicted.

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