Figure 2 | Scientific Reports

Figure 2

From: Transport of ultrasmall gold nanoparticles (2 nm) across the blood–brain barrier in a six-cell brain spheroid model

Figure 2

Six cell-type brain spheroid characterization. Immunohistochemistry was conducted on brain spheroids to determine phenotypic protein expression. The spheroids were stained for either ZO-1 or CD31 alone (bottom row) or combined with DAPI nuclear stain (upper row). The selected area was imaged at higher magnification to visualize the cell border connections formed by ZO-1 (B,E). The CD31 expression shows the localization of HBMEC in the brain spheroids. Images were obtained at × 10 and × 60 (B,E) magnification. Scale bars 30 μm and 200 μm (for × 10 and × 60, respectively).

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