Table 1 Parameters of gold nanoparticles applied to 3D cell culture models.

From: Transport of ultrasmall gold nanoparticles (2 nm) across the blood–brain barrier in a six-cell brain spheroid model

c(Au stock dispersion)/g/L

0.30

V(medium) per well/µL

100

V(Au stock dispersion) per well/µL

5

m(Au) per well/µg

1.50

c(Au) in a well/g/L

0.014

Particle molar concentration (d = 2 nm) in each well/µmol/L

0.29

Particle number concentration (d = 2 nm) in each well/L

1.73 × 1017

Particle number (d = 2 nm) in each well

1.82 × 1013

Dye molecules in each well

1.64 × ·1014

  1. For dissolved FAM-alkyne as control, the same molecular concentrations were used. The gold nanoparticles had a diameter of 1.57 ± 0.62 nm by DCS (lower than the core diameter due to the special characteristics of DCS with ultrasmall particles; see Ref.54), of 2.28 ± 0.32 nm by TEM, and carried 9 FAM molecules per nanoparticle. Dynamic light scattering (DLS) was not possible due to the small particle size. Based on 1H-DOSY results of similar nanoparticles, we estimate the hydrodynamic diameter to 3–4 nm (see Ref.30 for a full characterization of the particles).
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