Figure 6 | Scientific Reports

Figure 6

From: Addition of anaerobic electron acceptors to solid media did not enhance growth of 125 spacecraft bacteria under simulated low-pressure Martian conditions

Figure 6

Exposure of four spacecraft strains to low-PTA conditions on different medium types. The four species were Staphylococcus equorum subsp. equorum, Acinetobacter johnsonii, Sphingomonas yunnanensis, and Bacillus simplex. The media tested were TSA + vitamins, TSA + sulfate, and TSA + Fe3+ at pH 7, in which plates were incubated at low-PTA conditions for up to 28 days. Samples were taken every 7 days. Cells were applied on agar plates as 2.5-µL drops (~ 2.5 × 105 cells/drop), squares of agar/cells were cut out, and subsequent MPN assays completed. The data are reported as log 10 (N/N0) in which N0 = viable cell number on at T = 0 time, and N = viable cell numbers at specific points after incubation from 7 to 28 days (n = 6; error bars are standard deviations).

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