Figure 3

Cholesterol efflux activity of RG33 in lipid-free (LF) and lipid-bound (LB) states. (a) Concentration dependence of cholesterol efflux from 3H-cholesterol-labeled J774 cells by lipid-free (LF) and lipid-bound (LB) RG33 was compared after 2 h, n = 3. The maximum rate (Vmax) (b) and the Michaelis constant (Km) (c) were calculated using the Michaelis–Menten equation. GraphPad Prism (version 8.0) (www.graphpad.com) was used. Values are means ± SEM, ***p ≤ 0.001, ****p ≤ 0.0001.