Figure 4

RG33 improves glucose tolerance in GTT of DIO mice when administered by intraperitoneal or subcutaneous injection. (a) Male C57BL/6 mice fed High-Fat Diet for two weeks were fasted overnight, then treated for 3 h with a single intraperitoneal injection (i.p.), or subcutaneous injection (subQ) of NaCl (negative control, 200 µl, n = 6), or RG33 (12 mg/kg, n = 6). Mice then received an i.p. glucose load (40 mg/mouse) followed by blood and plasma collection at the indicated times and blood glucose (b, c) and insulin (e, f) concentrations determined for both injection routes. Total AUC glucose and AUC insulin were calculated (mmol/l x min and μg/l x min, respectively) then normalised to the average values of the relevant NaCl control groups to give relative AUC (% control) for glucose (d) and insulin (g). Data for NaCl control groups are adapted from15. GraphPad Prism (version 8.0) (www.graphpad.com) was used. Values are means ± SD, ***p ≤ 0.001, ****p ≤ 0.0001 versus NaCl control.