Figure 4 | Scientific Reports

Figure 4

From: A large deletion on CFA28 omitting ACSL5 gene is associated with intestinal lipid malabsorption in the Australian Kelpie dog breed

Figure 4

Sashimi plot of RNAseq data for CanFam3 genomic coordinates CFA28:23320000-23500000. The coverage for each alignment track is plotted as a bar graph, the Y axis represents read counts. Arcs are supported exon junctions and reads split across the junction (junction depth). Below the plots are the gene annotations for corresponding genomic coordinates. The figure illustrates RNAseq data from the jejunum of two samples; A case sample (AK Australian Kelpie) and control (LR Labrador retriever). Underlying DNA contamination can be seen in the AK highlighted by the low read count across the genomic region. A 103.3 kb deletion is seen in the AK, illustrated with a transparent box. The gap in the AK includes GUCY2GP, ACSL5, ZDHHC6 and a Long non-coding RNA (lncRNA). In the AK, expression of novel exons can be seen 148.4 kb downstream from the ZDHHC6 gene, the junction is supported by 152 reads. A consensus sequence produced using a genome guided de-novo assembly with Trinity is included in the gene track as ZDHHC6_AK_DEL.

Back to article page