Figure 2
From: p39-associated Cdk5 activity regulates dendritic morphogenesis
Impaired dendritic morphogenesis in Cdk5−/−, p39−/−, and p35−/− neurons. (a–d) Dendritic morphology of Cdk5+/+ and Cdk5−/− hippocampal neurons at 14 days in vitro (DIV). Representative images (a) and quantification of total dendrite number (b) and total dendrite length (c), and Sholl analysis (d) of Cdk5+/+ and Cdk5−/− neurons. Scale bar: 10 µm; n = 7–8 neurons; **p < 0.01, Mann–Whitney U-test. (e–h) Dendritic morphology of p39+/+ and p39−/− hippocampal neurons at 14 DIV. Representative images (e) and quantification of dendrite number (f) and total dendrite length (g), and Sholl analysis (h) of p39+/+ and p39−/− neurons. Scale bar: 10 µm; n = 30 neurons; ***p < 0.001, Mann–Whitney U-test. (i–l) Dendritic morphology of p35+/+ and p35−/− hippocampal neurons at 14 DIV. Representative images (i) and quantification of dendrite number (j) and total dendrite length (k), and Sholl analysis (l) of p35+/+ and p35−/− neurons. Scale bar: 10 µm; n = 7–8 neurons. (m–o) Dendritic morphology of pyramidal neurons in the cerebral cortex of 1-month-old p39+/+ and p39−/− mice. Representative images (m) and quantification of dendrite number (n) and total dendrite length (o). Scale bar: 50 µm; n = 22–29 neurons from 3 mice; ***p < 0.001, Mann–Whitney U-test.
