Figure 5
From: The HIF1α/JMY pathway promotes glioblastoma stem-like cell invasiveness after irradiation
Irradiation increases cellular levels of F-actin in a JMY-dependent manner. (A,C) F-actin staining with phalloidin in TG1N (A) or in TG16 (C) GSCs. Scale bars: 20 µM (A) and 10 µM (C). (B,D) Quantification of phalloidin fluorescence intensity 24 h after 0.5 Gy irradiation (in cells pretreated or not with 50 µM YC1) or after 100 µM DFO for TG1N (B) and TG16 GSCs (D). At least 35 cells were scored per condition (***p < 0.001). (E,F) Quantification of F-actin fluorescence intensity staining by phalloidin after irradiation (0.5 Gy) or not in GSCs with siCt, siJMY or siHIF1α-electroporated TG1N (E) and TG16 (F). At least 20 and 25 cells were scored by condition (respectively in TG1N and TG16 GSCs; *p < 0.05, ***p < 0.001 and ns not significant).
