Table 2 Intraday extraction and detection reproducibility for SPMs isolated from independent blood samples (n = 5).

From: The relationship between specialized pro-resolving lipid mediators, morbid obesity and weight loss after bariatric surgery

Compound

LODa (pg per injection)

LOQb (pg per injection)

d4-LTB4

0.5

1.0

d4-PGE2

0.5

1.0

d5-LXA4

0.1

0.2

d5-MaR2

1.0

2.0

d5-RvD2

0.1

0.2

d8-5-HETE

0.1

0.1

14-HDHA

0.1

0.1

17-HDHA

0.1

0.2

18-HEPE

0.5

1.0

LTB4

0.5

1.0

LXA4

0.1

0.2

LXB4

0.1

0.2

MaR1

0.2

0.5

MaR2

0.5

1.0

PD1

0.1

0.5

PDX

0.1

0.2

PGD2

0.1

0.1

PGE2

0.1

0.1

RvD1

0.2

0.5

RvD2

0.2

0.5

RvD3

0.1

0.2

RvD4

0.1

0.1

RvD5

0.1

0.1

RvE1

0.2

0.5

  1. d deuterium, LTB4 leukotriene B4, LXA4 lipoxin A4, LXB4 lipoxin B4, MaR1 maresin 1, MaR2 maresin 2, PD1 protectin D1, PDX protectin X, PGD2 prostaglandin D2, PGE2 prostaglandin E2, RvD1 resolvin D1, RvD2 resolvin D2, RvD3 resolvin D3, RvD4 resolvin D4, RvD5 resolvin D5, RvE1 resolvin E1, SPM specialized pro-resolving lipid mediators, 14-HDHA 14-hydroxy-docosahexaenoic acid, 17-HDHA 17-hydroxy-docosahexaenoic acid, 18-HEPE 18-hydroxy-eicosapentaenoic acid.
  2. aLimit of detection.
  3. bLimit of quantitation.
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