Figure 2 | Scientific Reports

Figure 2

From: Optimization of T4 phage engineering via CRISPR/Cas9

Figure 2

Doench scoring is not a good predictive measurement for selecting effective crRNAs. Experimental efficacy of DNA cleavage via EOP as evaluated in the soc, hoc, gp36, and gp38 genes in T4 phages does not align with theoretical projection. (A) Doench scoring (0–1) to theoretically assess crRNAs on-target activity and off-target sites in which values closer to 1 resemble the most potent crRNA. The crRNAs are ranked in descending efficacy order. (B) Validation of the crRNAs’ theoretical selection via plaque assay as demonstrated by the EOPs for soc, hoc, gp36, and gp38. Error bars indicate standard deviation of three experimental replicates. Lower EOP value represents better T4 phage DNA cleavage.

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