Figure 3 | Scientific Reports

Figure 3

From: Optimization of T4 phage engineering via CRISPR/Cas9

Figure 3

nluc insertion in T4 phages provides visual screening for recombinant phages. Comparing the efficiency of homologous recombination versus CRISPR/Cas9 assisted recombination. The schematic outlines the double plaque assay of T4 phage infection (A) of homologous recombination and (B) of CRISPR/Cas9 assisted recombination (representative images shown). The Nano-Glo luciferase assay system on the right demonstrates successful engineering of T4 phages (C) for homologous recombination (arrow) and (D) for CRISPR/Cas9-assisted recombination.

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