Figure 2 | Scientific Reports

Figure 2

From: The Fuchs corneal dystrophy-associated CTG repeat expansion in the TCF4 gene affects transcription from its alternative promoters

Figure 2

The TCF4 CTG trinucleotide repeat region modulates the activity of proximal downstream TCF4 promoters in a length dependent manner in neurons. (A,B) Promoter activities of TCF4 p4a (A) and TCF4 p4abc (B) constructs with different CTG repeat sizes (11, 25, 31, 54, 67 or 70, 144). Promoter activity was measured in rat cultured cortical neurons transfected with luciferase reporter constructs. Three independent experiments were performed for each promoter construct. A negative control (NC, promoterless pGL4.15 vector) is also shown. Statistical analysis was performed using one-way repeated-measures analysis of variance (ANOVA) with Greenhouse–Geisser correction followed by Dunnett’s post hoc test. Statistical significance is shown compared to the construct with 11 CTG repeats (*p < 0.05, **p < 0.01). Luciferase activity is shown relative to the luciferase signal obtained with the respective promoter construct with 11 CTG repeats.

Back to article page