Table 4 NormFinder analysis was presented.

From: The pseudogene problem and RT-qPCR data normalization; SYMPK: a suitable reference gene for papillary thyroid carcinoma

Analysis type

Tissue

Candidated genes

Stability value

Intragroup

Normal

GAPDH

1.941

TBP

1.030

SYMPK

0.704

PYCR1

1.305

ACTB

0.747

HPRT1

0.667

B2M

1.013

GUSB

0.745

PTC

GAPDH

1.416

TBP

1.295

SYMPK

0.458

PYCR1

0.754

ACTB

0.524

HPRT1

0.856

B2M

1.237

GUSB

0.421

Intergroup

Normal vs. PTC

GAPDH

0.698

TBP

0.551

SYMPKa

0.319

PYCR1

0.436

ACTB

0.395

HPRT1a

0.337

B2M

0.432

GUSBa

0.301

Best combination of two genes

SYMPK and ACTB

0.209

  1. NormFinder analysis was used to rank the candidate reference genes. NormFinder considered HPRT1 and GUSB as the best reference genes for normal and PTC tissues respectively, while SYMPK was the second most stable reference gene in both tissue types. Intergroup analysis indicated SYMPK and ACTB, with stability value of 0.209, as the best combination of reference genes.
  2. aGUSB, SYMPK, and HPRT1 were the best according to the Normfinder algorithm.
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