Figure 3
From: Deep learning method for comet segmentation and comet assay image analysis
Workflow of comet scoring procedure. First, each comet is extracted with its bounding box. If the background brightness is irregular, it is subtracted from the comet intensity. If the tails are not horizontal, the orientation is corrected for all the comets. Then, the head and tail are separated using the two predicted key points of the comet head, marked by a circled cyan mask. Finally, comets are scored based on the parameters, as shown in the sample table.
