Figure 2 | Scientific Reports

Figure 2

From: Salinomycin induces autophagic cell death in salinomycin-sensitive melanoma cells through inhibition of autophagic flux

Figure 2

Apoptosis induced by salinomycin in SK-Mel-19 cells. (a) Cells treated with 1.0 μM Sal were stained with Annexin V-FITC/PI and detected by flow cytometry analysis. (b) Levels of protein expression were measured by western blot analysis using antibodies against PARP1, Caspase9, and Caspase3. (c) Samples treated with Sal alone (1.0 μM, 24 h), or with Sal (1.0 μM) and zVAD-FMK (20 μM) for 24 h immunoblotted for cleaved-Caspase3. (d) Cell viability of SK-Mel-19 cells treated with different concentrations of Sal alone for 24 h or Sal combination with zVAD-FMK (20 μM) for 24 h was measured by MTS assay.

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