Figure 5 | Scientific Reports

Figure 5

From: Salinomycin induces autophagic cell death in salinomycin-sensitive melanoma cells through inhibition of autophagic flux

Figure 5

Failure of the fusion between autophagosomes and lysosomes. (a) Live-cell confocal microscopic images of SK-Mel-19-GFP-LC3B cells stained with LysoTracker Red treated with 1.0 μM Sal for 6 h or 12 h or 18 h (magnification, × 630). (b) Live-cell confocal microscopic images of SK-Mel-19-GFP-LC3B cells stained with LysoTracker Red treated with 1.0 μM Sal for 18 h, or treated with CQ for 6 h, or Rap for 6 h (magnification, × 630). (c) TEM images showing many autophagosomes, large-scale autophagic vacuolization of the cytoplasm and resultant vacuolated appearance in SK-Mel-19 cells after treated with Sal (1.0 μM) for 36 h.[Scale bars, 2 μm (Above) and 500 nm (Below).] (d, e) Bars denoted cell viability of SK-Mel-19 cells treated with Sal (0.5 μM, 1.0 μM) alone or combination with 3-MA (2.5 mM) or CQ (6.25 μM) respectively (**p < 0.01; ***p < 0.001).

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