Figure 6
Induction of ER stress and mitochondrial dysfunction in SK-Mel-19 cells. (a) EM images showing dilated ER and accumulation of smooth ER in 1.0 μM Sal-treated SK-Mel-19 cells.[Scale bars, 2 μm (Above) and 500 nm (Below).] (b) Samples treated with 1.0 μM Sal for the indicated period of time immunoblotted for ER stress related proteins. (c) Changes in JC-1 green and red fluorescence in SK-Mel-19 cells treated with Sal for 12 h or 24 h were detected by flow cytometer. The percentage of cells with low ΔΨm was determined (*p < 0.05; n = 3). (d) EM images showing abnormally looking mitochondria with fewer and swollen cristae in 1.0 μM Sal-treated SK-Mel-19 cells. [Scale bars, 2 μm (Above) and 500 nm (Below).] (e) The number of functional mitochondria and average size of each mitochondrion were quantified, n = 5–6 micrographs per condition (*p < 0.05).
