Figure 3

The twenty most abundant miRNAs, represented as reads per million, found in undifferentiated (a) and differentiated (b) Caco-2 cells, and in enteroids (isolated from male and female C57BL/6 mice) exposed to postprandial micelles (PPM) for 24 h (c). (d) Relative expression levels of selected miRNAs in differentiated Caco-2 cells exposed to DMEM (controls), empty micelles (EM) or PPM, for 24 h; n = 6 per group. One-way ANOVA was followed by Bonferroni’s post-hoc tests for multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.001. (e) Relative expression of selected miRNAs in intestinal organoids exposed to DMEM (controls; white), EM (grey) or PPM (dark grey), for 24 h; n = 4 per group. Two-way ANOVA was followed by Bonferroni’s post-hoc tests for multiple comparisons. *p < 0.05, **p < 0.01. (f,g) Light microscope representative images of mature enteroids treated with empty (f) or post-prandial (g) micelles (for each case, image on the left (10 ×) is zoomed on the right (20 ×)).