Table 2 Antibodies.
From: Hypoxia-inducible factor-2α is crucial for proper brain development
Visualisation of | Primary antibody | Secondary antibody |
|---|---|---|
Astrocytes | Glial fibrillary acidic protein (GFAP) – (mouse, #MAB360, Millipore) | M.O.M.™ anti-mouse IgG biotinylated – (#BMK2202. Vector Laboratories) |
HIF-1α | Hypoxia-inducible factor-1α – (rabbit, #10,006,421, Cayman Chemicals) | Anti-rabbit biotinylated –(#E043201, DAKO) |
HIF-2α | Hypoxia-inducible factor-2α – (rabbit, #NB100-122, Novus) | Anti-rabbit biotinylated –(#E043201, DAKO) |
Oligodendrocytes | Myelin basic protein (MBP) – (rabbit, #M3821, Sigma-Aldrich) | Anti-rabbit biotinylated –(#E043201, DAKO) |
Oligodendrocytes | Oligodendrocyte Marker O4 – (mouse, # MAB1326, R&D Systems) | Alexa 488 Goat-anti-mouse – (#A11001, Invitrogen) |
Neurons | Β(III)-Tubulin (rabbit, T2200, Sigma-Aldrich) | Alexa 488 Goat-anti-rabbit – (#A11034, Invitrogen) |
Pyramidal cells | Neurogranin (NRGN) – (rabbit, #10,440–1-AP, proteintech™) | Anti-rabbit biotinylated –(#E043201, DAKO) |
