Figure 3
From: ESCRT-III controls nuclear envelope deformation induced by progerin
Effects of ectopic expression of CHMP4B in HeLa cells. (a) HeLa/puro, HeLa/CHMP4BKO/puro and HeLa/CHMP4BKO/CHMP4B-EGFP cells were transfected with the TagRFP-progerin expression vector for 24 h. The number of nuclear tube-like structures was measured in 100 transfected cells. Data are shown as the mean ± SEM and are representative of 3 independent experiments. (b) Lysates of HeLa/puro, HeLa/CHMP4BKO/puro and HeLa/CHMP4BKO/CHMP4B-EGFP cells transfected with the TagRFP-progerin expression vector for 24 h were analyzed by immunoblotting with anti-TagRFP, anti-CHMP4B and anti-α-tubulin antibodies. Images are representative of 3 independent experiments. Full-length blots are presented in Supplementary Fig. S2.
