Figure 6

Effect of rapamycin treatment in ESCRT-III depleted cells. (a) Confocal microscope images of HeLa cells and HeLa/CHMP4BKO cells were transfected with the plasmid expressing TagRFP-progerin for 24 h followed by mock or rapamycin treatment in the presence or absence of Bafilomycin A1 (Baf-A1) for 24 h. Bars, 20 µm. Images are representative of 3 independent experiments. (b) Relative number of trans-nuclear tubes of HeLa cells and HeLa/CHMP4BKO cells transfected with the plasmid expressing TagRFP-progerin, followed by the rapamycin treatment in the presence or absence of Baf-A1 to these of mock treated cells as described in (a). Data are shown as the mean ± SEM of 3 independent experiments. The indicated p values were obtained using Tukey’s test. n.s. not significant.