Figure 2
ARG diversity and clustering in vertebrate samples across habitats. (a) Species richness. (b) Shannon diversity index. (c) Simpson diversity index. The horizontal box lines represent the first quartile, the median, and the third quartile. Whiskers denote the range of points within the first quartile − 1.5 × the interquartile range and the third quartile + 1.5 × the interquartile range. Diamond represents the mean values. Differences between groups were estimated using a Kruskal–Wallis test. (d–e) Non-metric multidimensional scaling (NMDS) analysis on ARG type (d) and gene (e) levels. Ellipses were drawn at a confidence level of 0.95. Effect of habitat on resistome clustering was assessed using an Adonis test. (f) Heat map on ARG type level. Bray–Curtis dissimilarity and Pearson correlation coefficients were used to hierarchically cluster (using the UPGMA method) samples and ARGs, respectively.
