Figure 1

Detection of CoTS DNA in fish faecal and gut content samples. Examples for positive and negative digital droplet PCR results for four different coral reef fish, namely Banded Humbug (Dascyllus aruanus; positive), Blackvent Damsel (Dischistodus melanotus; negative), Spangled Emperor (Lethrinus nebulosus; positive), and Common Coral Trout (Plectropomus leopardus; positive). Sample collection number for each individual fish are given. Examples of digital droplet PCR results for positive (one to two 8-day old Acanthaster cf. solaris larvae) and negative (blanks) controls are also provided.