Figure 3 | Scientific Reports

Figure 3

From: Synthesis of two new lipid mediators from docosahexaenoic acid by combinatorial catalysis involving enzymatic and chemical reaction

Figure 3

RP-HPLC analysis of epoxide ring-opened products by sodium hydroxide (NaOH). (a) Purified 7S,15R-dihydroxy-16S,17S-epoxy-docosapentaenoic acid as a precursor. (b) Two products were generated by NaOH: main product was 7S,15R,16S,17S-tetrahydroxy docosapentaenoic acid (conversion rate of 90% or less) and minor product was RvD2 (conversion rate of 10%). (c) Tetrahydroxy fatty acid was produced by epoxide hydrolase (EH; commercially available) to use as a reference. (d) RvD2 standard. The epoxide ring-opening reaction was performed with NaOH at 30 °C overnight.

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