Figure 1 | Scientific Reports

Figure 1

From: Hic-5 is required for activation of pancreatic stellate cells and development of pancreatic fibrosis in chronic pancreatitis

Figure 1

Hic-5 expression is enhanced in CP and in activated PSCs. (A) Immunohistochemical staining of Hic-5 in normal human pancreatic tissue (left) and chronic pancreatitis (CP) tissue (right); magnification, × 100 (top), × 400 (bottom). (B) Double immunofluorescence staining of Hic-5 (red) and α-SMA (green) in human pancreatic tissue with CP; nucleus (blue). Scale bars indicate 50 μm (C) Double immunofluorescence staining of Hic-5 (red) and α-SMA (green) in mouse pancreatic tissue with CP; nucleus (blue). Scale bars indicate 50 μm (D) Western blot analysis of Hic-5 expression in mouse pancreas from WT mice with or without caerulein treatment. (E) mRNA expression of Hic-5 in mouse pancreas with or without caerulein-treatment. (F) Double immunofluorescence staining of Hic-5 (red) and α-SMA (green) in cultured PSCs from WT mice at the indicated time points. Scale bars indicate 20 μm (G) Western blot analysis of Hic-5 and α-SMA expression in cultured PSCs from WT mice at the indicated time points. (H) mRNA expression of Hic-5 in cultured PSCs from WT mice at the indicated time points. *p < 0.05.

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