Figure 2 | Scientific Reports

Figure 2

From: Hic-5 is required for activation of pancreatic stellate cells and development of pancreatic fibrosis in chronic pancreatitis

Figure 2

Hic-5 deficiency reduces fibrosis development in caerulein-induced CP. (A) Determination of the ratio of pancreatic tissue weight to mouse body weight. (B) Mouse pancreatic tissues examined by H&E staining. WT (top left × 100), Hic-5 KO (bottom left × 100), WT CP (upper × 100, top right × 400) and Hic-5 KO CP (lower × 100, lower right × 400). (C) Sirius Red staining and Masson’s trichrome staining of mouse pancreatic tissue. (D) Determination of the ratio of the Sirius Red stained area to total tissue size. (E) The expression of Collagen I and α-SMA was detected by western blotting. (F) Quantitative analysis of Collagen I and α-SMA expression (E) after normalization against GAPDH. (G) mRNA analysis of Col1a1, Col3a1, and α-sma expression. *p < 0.05.

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