Figure 3 | Scientific Reports

Figure 3

From: Hic-5 is required for activation of pancreatic stellate cells and development of pancreatic fibrosis in chronic pancreatitis

Figure 3

Hic-5 deficiency attenuates activation of cultured mouse PSCs by impairing Smad2 phosphorylation. (A) The expression of Col1a1 and α-SMA mRNA in PSCs from the two groups was detected by RT-qPCR. (B) The expression of Hic-5 and α-SMA in PSCs from the two groups was detected by western blotting at 2 h and at 5 days. (C) Detection of Hic-5 (red) and α-SMA (green) expression by immunofluorescence. The upper panels show PSCs isolated from WT mice. The lower panels show PSCs isolated from Hic-5 KO mice. Scale bars indicate 20 μm (D) The expression of p-Smad2 and Hic-5 was detected by western blotting. (E) Quantitative analysis of p-Smad2 is shown. *p < 0.05.

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